Mouse decay-accelerating factor: selective and tissue-specific induction by estrogen of the gene encoding the glycosylphosphatidylinositol-anchored form.

摘要: Neonatal exposure of mice to estrogen (diethylstilbestrol) results in a high incidence (90%) uterine tumor later life. In an effort screen for estrogen-regulated genes the uterus neonatal mouse, we have isolated murine homologue human decay-accelerating factor (DAF), glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein and member regulators complement activation family proteins that function prevent autologous complement-mediated tissue damage. The induced mouse DAF cDNA has 64% sequence identity with counterpart at nucleotide level 50% deduced amino acid sequence. It consists 390 acids contains four short consensus repeats internal homology characteristic DAF. also hydrophobic C-terminal most likely serves as signal GPI anchor attachment. Sequence comparison recently reported cDNAs confirmed estrogen-inducible gene corresponds gene. induction by is specific can be mimicked antiestrogen tamoxifen. Furthermore, regulation expression limited transmembrane not expressed uterus, either or without stimulation. These suggest two are differentially regulated, GPI-anchored may play important roles responses other physiologic pathophysiologic processes female reproductive system.