Physiological and epigenetic analyses of Brassica napus seed germination in response to salt stress

摘要: Salinity stress significantly affects plant growth and development because of osmotic stress, ion toxicity, nutrient imbalance. Therefore, salinity becomes a serious threat to rapeseed production in agriculture. Plants evolved series complex mechanisms, including morphological changes, physiological adjustment, gene expression regulation, at molecular level adapt salt stress. Epigenetic regulations, DNA methylation histone modification, play major role tuning response environmental stimuli. Although many progresses have been reported the epigenetic changes Brassica napus under are far from being understood. A parameters, water content, proline malondialdehyde electrolyte leakage, antioxidant enzyme activities, different concentrations (0, 25, 50, 100 mM) NaCl treatment “Yangyou 9” was determined germination stage. Immunofluorescent staining high-performance liquid chromatography-assisted quantification were conducted analyze distribution patterns Results analyses indicated that 25 mM promoted seedlings, whereas 50 100 mM treatments inhibited seedlings. investigations showed mediated enrichment H3K4me3, as well decreases H3K9me2 5-methylcytosine (5-mC), induced increases 5-mC decrease H3K4me3. Overall, this study offers new insights into rapeseed, information would be propitious engineer crops with enhanced tolerance.