Glucose biosensor based on platinum microparticles dispersed in nano-fibrous polyaniline

作者: Haihui Zhou , Hong Chen , Shenglian Luo , Jinhua Chen , Wanzhi Wei

DOI: 10.1016/J.BIOS.2004.04.024

关键词: AmperometryEnzyme electrodeScanning electron microscopeMaterials sciencePlatinumNuclear chemistryBiosensorOrganic chemistryGlucose oxidasePolyanilineAscorbic acid

摘要: A sensitive and selective amperometric glucose biosensor based on platinum microparticles dispersed in nano-fibrous polyaniline (PANI) was investigated. Poly (m-phenylenediamine) (PMPD), which employed as an anti-interferent barrier a protective layer to microparticles, deposited onto platinum-modified PANI the presence of oxidase. The morphology PANI, Pt/PANI PMPD-GOD/Pt/PANI were investigated by scanning electron microscopy. results show that has morphology. enzyme electrode exhibits excellent response performance with linear range from 2 x 10(-6) 12 10(-3) M fast time within 7s. Due permeability PMPD, also shows good anti-interference uric acid ascorbic acid. Michaelis-Menten constant km maximum current density imax 9.34 917.43 microA cm(-2), respectively. Furthermore, this stability reproducibility.

参考文章(28)
A Kelaidopoulou, A Papoutsis, G Kokkinidis, WT Napporn, J-M Leger, C Lamy, None, Electrooxidation of β-D(+)glucose on bare and u.p.d. modified platinum particles dispersed in polyaniline Journal of Applied Electrochemistry. ,vol. 29, pp. 101- 107 ,(1999) , 10.1023/A:1003433206439
Hitoshi Yamato, Takafumi Koshiba, Masaki Ohwa, Wolfgang Wernet, Michio Matsumura, A new method for dispersing palladium microparticles in conducting polymer films and its application to biosensors Synthetic Metals. ,vol. 87, pp. 231- 236 ,(1997) , 10.1016/S0379-6779(97)80114-2
P. Nunziante, G. Pistoia, Factors affecting the growth of thick polyaniline films by the cyclic voltammetry technique Electrochimica Acta. ,vol. 34, pp. 223- 228 ,(1989) , 10.1016/0013-4686(89)87089-6
G. Zotti, S. Cattarin, N. Comisso, Cyclic potential sweep electropolymerization of aniline: The role of anions in the polymerization mechanism Journal of Electroanalytical Chemistry. ,vol. 239, pp. 387- 396 ,(1988) , 10.1016/0022-0728(88)80293-6
Mirtha. Umana, Jess. Waller, Protein-modified electrodes. The glucose oxidase/polypyrrole system Analytical Chemistry. ,vol. 58, pp. 2979- 2983 ,(1986) , 10.1021/AC00127A018
P.N. Barlett, J.M. Cooper, A review of the immobilization of enzymes in electropolymerized films Journal of Electroanalytical Chemistry. ,vol. 362, pp. 1- 12 ,(1993) , 10.1016/0022-0728(93)80001-X
Hitoshi Muguruma, Atsunori Hiratsuka, Isao Karube, Thin-Film Glucose Biosensor Based on Plasma-Polymerized Film: Simple Design for Mass Production Analytical Chemistry. ,vol. 72, pp. 2671- 2675 ,(2000) , 10.1021/AC000014N
Haesik Yang, Taek Dong Chung, Youn Tae Kim, Chang Auck Choi, Chi Hoon Jun, Hee Chan Kim, Glucose sensor using a microfabricated electrode and electropolymerized bilayer films. Biosensors and Bioelectronics. ,vol. 17, pp. 251- 259 ,(2002) , 10.1016/S0956-5663(01)00266-4
C Coutanceau, M.J Croissant, T Napporn, C Lamy, Electrocatalytic reduction of dioxygen at platinum particles dispersed in a polyaniline film Electrochimica Acta. ,vol. 46, pp. 579- 588 ,(2000) , 10.1016/S0013-4686(00)00641-1