Differential stabilities of soil enzymes

摘要: Abstract Methods have been refined for the assay of phosphatase and arylsulphatase activities in soil, based on chromogenic p -nitrophenyl ester substrates. Basic conditions defined, pH optima kinetic parameters determined. The enzymes follow Michaelis-Menten kinetics; this conclusion is three methods analysis data determined over a wide range substrate concentrations. enzyme are very stable to storage wet soil up 4 weeks at temperatures above. For example, had half-life approximately 2 50°C; was rather less stable. Both retained 80% activity after incubation with pronase 1 week 25°C. On basis work studies other enzymes, it concluded that remarkable stability general feature enzymes.