Transcriptional Regulation of the β-Type Carbonic Anhydrase Gene bca by RamA in Corynebacterium glutamicum
作者： Adnan Shah , Bernhard J. Eikmanns
DOI: 10.1371/JOURNAL.PONE.0154382
关键词: Start codon 、 Regulation of gene expression 、 Gene expression 、 Biochemistry 、 Biology 、 Molecular biology 、 Corynebacterium glutamicum 、 Mutant 、 Promoter 、 Carbonic anhydrase 、 Gene
摘要: Carbonic anhydrase catalyzes the reversible hydration of carbon dioxide to bicarbonate and maintains balance CO2/HCO3- in intracellular environment, specifically for carboxylation/decarboxylation reactions. In Corynebacterium glutamicum, two putative genes, namely bca (cg2954) gca (cg0155) coding β-type γ-type carbonic anhydrase, respectively, have been identified. We here analyze transcriptional organization these genes. The start site (TSS) gene was shown be first nucleotide “A” its translational codon (ATG) thus, codes a leaderless transcript. TSS identified as an residue located at position -20 relative annotated cg0154 gene, which is immediately upstream gca. Comparative expression analysis revealed source-dependent regulation with 1.5- 2-fold lower promoter activity cells grown on acetate compared glucose sole source. Based higher mutant deficient regulator metabolism RamA wild-type C. glutamicum based binding His-tagged protein region, we present evidence that negatively regulates glutamicum. Functional characterization deletion same growth characteristics ∆gca no effect gene.
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sci-hub.st 参考文章(94)
P. Vasicová, Z. Abrhámová, J. Nesvera, M. Pátek, H. Sahm, B. Eikmanns, Integrative and autonomously replicating vectors for analysis of promoters in Corynebacterium glutamicum Biotechnology Techniques. ,vol. 12, pp. 743- 746 ,(1998) , 10.1023/A:1008827609914
Virendra S Bisaria, Akihiko Kondo, None, Bioprocessing of renewable resources to commodity bioproducts Published in <b>2014</b> by Wiley. ,(2014)
Jaide V.K. Jensen, Dorit Eberhardt, Volker F. Wendisch, Modular pathway engineering of Corynebacterium glutamicum for production of the glutamate-derived compounds ornithine, proline, putrescine, citrulline, and arginine Journal of Biotechnology. ,vol. 214, pp. 85- 94 ,(2015) , 10.1016/J.JBIOTEC.2015.09.017
Stefan Wieschalka, Bastian Blombach, Michael Bott, Bernhard J. Eikmanns, Bio-based production of organic acids withCorynebacterium glutamicum Microbial Biotechnology. ,vol. 6, pp. 87- 102 ,(2013) , 10.1111/1751-7915.12013
Robert K. Kulis‐Horn, Marcus Persicke, Jörn Kalinowski, Histidine biosynthesis, its regulation and biotechnological application in Corynebacterium glutamicum Microbial Biotechnology. ,vol. 7, pp. 5- 25 ,(2014) , 10.1111/1751-7915.12055
Michael Vogt, Sabine Haas, Tino Polen, Jan Ooyen, Michael Bott, Production of 2-ketoisocaproate with Corynebacterium glutamicum strains devoid of plasmids and heterologous genes. Microbial Biotechnology. ,vol. 8, pp. 351- 360 ,(2015) , 10.1111/1751-7915.12237
M B Guilloton, A F Lamblin, E I Kozliak, M Gerami-Nejad, C Tu, D Silverman, P M Anderson, J A Fuchs, A physiological role for cyanate-induced carbonic anhydrase in Escherichia coli. Journal of Bacteriology. ,vol. 175, pp. 1443- 1451 ,(1993) , 10.1128/JB.175.5.1443-1451.1993
Shigezo Udaka, Shukuo Kinoshita, Masakazu Shimono, Studies on the amino acid fermentation. Part 1. Production of L-glutamic acid by various microorganisms. Journal of General and Applied Microbiology. ,vol. 50, pp. 331- 343 ,(2004)
Koichi Toyoda, Masayuki Inui, Regulons of global transcription factors in Corynebacterium glutamicum. Applied Microbiology and Biotechnology. ,vol. 100, pp. 45- 60 ,(2016) , 10.1007/S00253-015-7074-3
Sabine A. E. Heider, Volker F. Wendisch, Engineering microbial cell factories: Metabolic engineering of Corynebacterium glutamicum with a focus on non-natural products. Biotechnology Journal. ,vol. 10, pp. 1170- 1184 ,(2015) , 10.1002/BIOT.201400590