Elucidating the impact of novel A1 and A2 Phytophthora infestans strains on existing Irish blight populations

摘要: The over-arching objective of this study was to explore the potential impact novel A1 and A2 isolates Phytophthora infestans on existing population in Ireland these new strains potato blight management efforts. Towards this, activities were executed determine P. structure genetic diversity afterwards investigate for changes populations through sexual recombination or host resistance associated adaptive change. Simple sequence repeat (SSR) marker based analysis sampled Irish (in 2009) revealed: (i) moderate (ii) two co-existing clusters delineating with mating type metalaxyl phenotypes (iii) on-going displacement clonal lineages by genotypes. In parallel, higher foliar aggressiveness four cultivars recorded genotypes compared those ‘traditional’ genotypes. SSR F1 progeny derived from crosses between revealed levels diversity, typically expected mating. However, be less fit a moderately resistant cultivar Cara parental isolates. Following several reiterative host-pathogen interactions, demonstrated adapt transgenic lines tested, which are equipped ‘resistance blight’ (RB) gene. Adapted showed amino acid substitutions carboxylic terminal avirulent ipiO protein, is complementary target RB protein. Separately, efforts develop quantitative PCR assay also explored culminating technique capable detecting asexual propagules infected tubers. In light emergence Ireland, provides valuable information both research community Irish potato sector as whole. This timely high pressure continuously throughout regards developing debate testing GM potatoes Ireland.