Homologue expression of a fungal endo-1,4-β-Dxylanase using submerged and solid substrate fermentations
作者： A Amaro-Reyes , BE García-Almendárez , DG Vázquez-Mandujano , S Amaya-Llano , E Castaño-Tostado
DOI: 10.4314/AJB.V10I10
关键词: Fermentation 、 Aspergillus niger 、 Dehydrogenase 、 Biochemistry 、 Recombinant DNA 、 Chemistry 、 Peptide 、 Solid-state fermentation 、 Enzyme 、 Chromatography 、 Expression cassette
摘要: The xyn5 gene, which encodes an endo-β-1,4-xylanase (Xyn5), in Aspergillus niger GS1 was cloned into expression cassette under the control of constitutive glyceraldhehyde-3-phosphate dehydrogenase gene promoter. system designed to produce recombinant enzyme containing a six-histidine peptide fused carboxyl end protein. efficiency Xyn5 production submerged (SmF) and solid-state (SSF) fermentation investigated using homologous cotransformed A. AB4.1. A productivity 17.1 U/(l·h) estimated for SSF 3.2 SmF calculated at peak value titers. Recombinant obtained by on polyurethane fiber, purified 5.1-fold anion exchange immobilized metal affinity chromatography, with 35.7% recovery. showed apparent molecular weight 30 kDa optimal activity (522 U/mg protein) pH 5.5 50°C. Key words: GS1, xylanolytic activity, fermentation, homologue expression, fiber.
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