THE PERMITTISTAT : A NOVEL TYPE OF TURBIDOSTAT
作者： G. H. Markx , C. L. Davey , D. B. Kell
DOI: 10.1099/00221287-137-4-735
关键词: Turbidity 、 Dry weight 、 Yeast 、 Turbidostat 、 Chromatography 、 Fermentation 、 Methylene blue 、 Permittivity 、 Analytical chemistry 、 Turbidimetry 、 Chemistry
摘要: Summary: Baker's yeast was grown in a novel type of turbidostat which the steady-state biomass level controlled not by optical turbidity but dielectric permittivity suspension at appropriate radio frequencies. Dry weight, fresh density 600 nm, percentage viability (from methylene blue staining), bud count and ethanol concentration were measured off-line cell size distribution recorded using flow cytometry. Any changes physiological properties had negligible effect on ratio between set (and measured) dry weight or cultures. The permittistat found to provide an extremely convenient means for carrying out turbidostatic culture.
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