Trapping of free radicals with direct in vivo EPR detection: a comparison of 5,5-dimethyl-1-pyrroline-N-oxide and 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide as spin traps for HO* and SO4*-.

作者: Graham S Timmins , Ke Jian Liu , Etelvino JH Bechara , Yashige Kotake , Harold M Swartz

DOI: 10.1016/S0891-5849(99)00049-0

关键词: PhotochemistryNucleophilic substitutionPyrrolineAdductChemistryElectron paramagnetic resonanceHydroxyl radicalRadicalIn vivoOxide

摘要: … Typical resultant EPR spectra using DMPO and DEPMPO as spin traps are shown in Figs. 1A and 1B respectively. All components of the system were required for its observation; with …

参考文章(25)
George Huntington Williams, Advances in free-radical chemistry Logos Press. ,(1965)
Ronald P. Mason, Kathryn T. Knecht, [10] In Vivo detection of radical adducts by electron spin resonance Methods in Enzymology. ,vol. 233, pp. 112- 117 ,(1994) , 10.1016/S0076-6879(94)33013-1
Jacques Reuben, Lawrence J. Berliner, Biological Magnetic Resonance ,(1982)
Hakim Karoui, Neil Hogg, Claudine Fréjaville, Paul Tordo, B. Kalyanaraman, Characterization of Sulfur-centered Radical Intermediates Formed during the Oxidation of Thiols and Sulfite by Peroxynitrite ESR-SPIN TRAPPING AND OXYGEN UPTAKE STUDIES Journal of Biological Chemistry. ,vol. 271, pp. 6000- 6009 ,(1996) , 10.1074/JBC.271.11.6000
R. Sridhar, P. C. Beaumont, E. L. Powers, Fast kinetics of the reactions of hydroxyl radicals with nitrone spin traps Journal of Radioanalytical and Nuclear Chemistry. ,vol. 101, pp. 227- 237 ,(1986) , 10.1007/BF02042423
K.T. Knecht, R.P. Mason, In Vivo Spin Trapping of Xenobiotic Free Radical Metabolites Archives of Biochemistry and Biophysics. ,vol. 303, pp. 185- 194 ,(1993) , 10.1006/ABBI.1993.1272
Michael J Davies, Bruce C Gilbert, Jonathan K Stell, Adrian C Whitwood, None, Nucleophilic substitution reactions of spin adducts. Implications for the correct identification of reaction intermediates by EPR/spin trapping Journal of The Chemical Society-perkin Transactions 1. pp. 333- 335 ,(1992) , 10.1039/P29920000333
Thierry Douki, Janice Onuki, Marisa HG Medeiros, Etelvino JH Bechara, Jean Cadet, Paolo Di Mascio, None, Hydroxyl radicals are involved in the oxidation of isolated and cellular DNA bases by 5-aminolevulinic acid FEBS Letters. ,vol. 428, pp. 93- 96 ,(1998) , 10.1016/S0014-5793(98)00504-3
Ke Jian Liu, Jin Jie Jiang, Li Li Ji, Xianglin Shi, Harold M. Swartz, An HPLC and EPR investigation on the stability of DMPO and DMPO spin adducts in vivo Research on Chemical Intermediates. ,vol. 22, pp. 499- 509 ,(1996) , 10.1163/156856796X00700
Ke Jian Liu, Minoru Miyake, Tomasz Panz, Harold Swartz, Evaluation of DEPMPO as a spin trapping agent in biological systems. Free Radical Biology and Medicine. ,vol. 26, pp. 714- 721 ,(1999) , 10.1016/S0891-5849(98)00251-2