Simultaneous determination of polyamines in human nail as 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole derivatives by nano-flow chip LC coupled with quadrupole time-of-flight tandem mass spectrometry.

摘要: Abstract Background Polyamines are active biogenic amines which play an important role in cell growth and proliferation the synthesis of proteins nucleosides. In recently studies, rapid tumor has been associated with markedly altered polyamine biosynthesis accumulation. Therefore, accurate detection identification all polyamines simultaneously a single analysis is becoming more for study their biochemical roles. Methods The development simultaneous determination method 9 human nails was attempted by combination nano-flow chip LC quadrupole time-of-flight tandem mass spectrometry (Q-TOF-MS/MS). proposed healthy persons cancer patients. Results derivatives nail were completely separated gradient 18 min duration wash program using reversed-phase column. then introduced into Q-TOF-MS/MS instrument sensitively detected ESI + mode. Polyamine concentration different based on gender, i.e., PUT SPD higher men than women persons. Additionally, lung patients group, as compared persons, concentrations PUT, N 1 -actPUT, SPM significantly increased. Conclusions We here present novel sensitive, simple nails. Furthermore, we show that (ORN, DAP, CAD, 8 -actSPD, -actSPM) from Human may serve noninvasive bio sample diagnosis chronic disease.