The correlation between inflammatory injury induced by LPS and RAS in EpH4-Ev cells
作者： Kun Wang , Xiaoqian Liu , Hang Xiao , Huanhuan Wang , Yuanshu Zhang
DOI: 10.1016/J.INTIMP.2017.02.016
关键词: MTT assay 、 Western blot 、 Interleukin 8 、 Viability assay 、 Inflammation 、 TLR4 、 Cell culture 、 Tumor necrosis factor alpha 、 Biology 、 Molecular biology
摘要: Renin-angiotensin system (RAS) plays an important role of regulating inflammatory injury. However, it is not clear about the correlation between renin-angiotensin and inflammation induced by LPS in mammary gland cells. So immunofluorescence was performed to verify ACE2 expression MTT assay detect cell viability. ELISA cytokines supernatant. Western Blot analyze RAS levels level change observed immunofluorescence. The TLR4 p65 phosphorylation were detected Blot. protein intensively located on membrane. According results TNF-α level, injury evidently high concentration after 9h. TNF-α, IL-6, IL-8, ACE, AT1R AngII had increasing with rise In contrast, MasR, Ang1-7 a declining increase degree. group significantly higher than that control group. These suggest valid cells treated for Meanwhile, ACE/AngII/AT1R axis activated ACE2/Ang1-7/MasR depressed.
sci-hub.se 参考文章(36)
Yi Luo, Eriko Tanabe, Misaho Kitayoshi, Yukiko Nishiguchi, Rina Fujiwara, Sayako Matsushima, Takamitsu Sasaki, Tomonori Sasahira, Yoshitomo Chihara, Dai Nakae, Kiyomu Fujii, Hitoshi Ohmori, Hiroki Kuniyasu, Expression of MAS1 in breast cancer. Cancer Science. ,vol. 106, pp. 1240- 1248 ,(2015) , 10.1111/CAS.12719
Mark C. Chappell, Nonclassical Renin‐Angiotensin System and Renal Function Comprehensive Physiology. ,vol. 2, pp. 2733- 2752 ,(2012) , 10.1002/CPHY.C120002
Yumiko Imai, Keiji Kuba, Shuan Rao, Yi Huan, Feng Guo, Bin Guan, Peng Yang, Renu Sarao, Teiji Wada, Howard Leong-Poi, Michael A. Crackower, Akiyoshi Fukamizu, Chi-Chung Hui, Lutz Hein, Stefan Uhlig, Arthur S. Slutsky, Chengyu Jiang, Josef M. Penninger, Angiotensin-converting enzyme 2 protects from severe acute lung failure Nature. ,vol. 436, pp. 112- 116 ,(2005) , 10.1038/NATURE03712
Yingchuan Li, Yongmei Cao, Zhen Zeng, Mengfan Liang, Ying Xue, Caihua Xi, Ming Zhou, Wei Jiang, Angiotensin-converting enzyme 2/angiotensin-(1–7)/Mas axis prevents lipopolysaccharide–induced apoptosis of pulmonary microvascular endothelial cells by inhibiting JNK/NF–κB pathways Scientific Reports. ,vol. 5, pp. 8209- 8209 ,(2015) , 10.1038/SREP08209
Javier Oviedo-Boyso, Juan J. Valdez-Alarcón, Marcos Cajero-Juárez, Alejandra Ochoa-Zarzosa, Joel E. López-Meza, Alejandro Bravo-Patiño, Víctor M. Baizabal-Aguirre, Innate immune response of bovine mammary gland to pathogenic bacteria responsible for mastitis. Journal of Infection. ,vol. 54, pp. 399- 409 ,(2007) , 10.1016/J.JINF.2006.06.010
Mary Donoghue, Frank Hsieh, Elizabeth Baronas, Kevin Godbout, Michael Gosselin, Nancy Stagliano, Michael Donovan, Betty Woolf, Keith Robison, Raju Jeyaseelan, Roger E. Breitbart, Susan Acton, A Novel Angiotensin-Converting Enzyme–Related Carboxypeptidase (ACE2) Converts Angiotensin I to Angiotensin 1-9 Circulation Research. ,vol. 87, ,(2000) , 10.1161/01.RES.87.5.E1
Shizuo Akira, Kiyoshi Takeda, Toll-like receptor signalling Nature Reviews Immunology. ,vol. 4, pp. 499- 511 ,(2004) , 10.1038/NRI1391
Toshihide Kurihara, Yoko Ozawa, Kei Shinoda, Norihiro Nagai, Makoto Inoue, Yuichi Oike, Kazuo Tsubota, Susumu Ishida, Hideyuki Okano, Neuroprotective effects of angiotensin II type 1 receptor (AT1R) blocker, telmisartan, via modulating AT1R and AT2R signaling in retinal inflammation Investigative Ophthalmology & Visual Science. ,vol. 47, pp. 5545- 5552 ,(2006) , 10.1167/IOVS.06-0478
Robson A. S. Santos, Ana C. Simoes e Silva, Christine Maric, Denise M. R. Silva, Raquel Pillar Machado, Insa de Buhr, Silvia Heringer-Walther, Sergio Veloso B. Pinheiro, Myriam Teresa Lopes, Michael Bader, Elizabeth P. Mendes, Virgina Soares Lemos, Maria Jose Campagnole-Santos, Heinz-Peter Schultheiss, Robert Speth, Thomas Walther, Angiotensin-(1–7) is an endogenous ligand for the G protein-coupled receptor Mas Proceedings of the National Academy of Sciences of the United States of America. ,vol. 100, pp. 8258- 8263 ,(2003) , 10.1073/PNAS.1432869100
Satoshi Hagiwara, Hideo Iwasaka, Shigekiyo Matumoto, Seigo Hidaka, Takayuki Noguchi, Effects of an angiotensin-converting enzyme inhibitor on the inflammatory response in in vivo and in vitro models Critical Care Medicine. ,vol. 37, pp. 626- 633 ,(2009) , 10.1097/CCM.0B013E3181958D91