DNA Methylation by DNA Methyltransferase 1 Is Critical for Effector CD8 T Cell Expansion
作者： Craig Chappell , Caroline Beard , John Altman , Rudolph Jaenisch , Joshy Jacob
DOI: 10.4049/JIMMUNOL.176.8.4562
关键词: DNA methylation 、 T cell 、 Biology 、 CD8 、 Cytotoxic T cell 、 IL-2 receptor 、 Cancer research 、 DNA methyltransferase 、 Interleukin 21 、 ZAP70 、 Molecular biology
摘要: Transcriptional silencing mediated by DNA methylation is a critical component of epigenetic regulation during early embryonic development in animals. However, the requirement for activation and differentiation mature CD8+ T cells into effector memory not clear. Using cre-mediated deletion methyltransferase 1 ( Dnmt1 ) at time cell activation, we investigated obligation maintaining patterns generation Ag-specific response to acute viral infection mice with lymphocytic choriomeningitis virus. −/− failed undergo massive expansion characteristic virus infection, leading >80% reductions height response. Despite this, efficiently controlled infection. Interestingly, number was moderately reduced compared seen day 8 postinfection. Our data suggest that ablation subsequent affect finite proliferative potential moderate effects on their cells.
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