Template size matched film thickness for effectively in situ surface imprinting: a model study of glycoprotein imprints

摘要: Precisely controlling the material structure is a high requirement for biological target imprinting. In situ surface imprinting immobilized templates can offer thin-film molecular imprints containing site-directed binding sites on substrates, and therefore appropriate targets. However, correlation between required film thickness superior effect bulk of template not clearly understood. Here we use series glycoprotein imprinted films as model to give semi-quantitative description their correlation. Glycoproteins with distinguished sizes including ribonuclease B, glucose oxidase horseradish peroxidase were used templates. Covalently immobilizing glycoproteins was achieved by using m-aminophenylboronic acid modified SiO2 or Fe3O4 surface. Dopamine polymerized onto this Varying polymerization time provided tunable in nanometer-scale. The isotherm study each different performed. optimal highest capacities factors shows positive its size. optimized recognize simple complex environment. These results suggest that should be tailored matching geometric size fixed templates, reveal substantial influence design.