Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis
作者： Shuai You , Chun-Chi Chen , Tao Tu , Xiaoyu Wang , Rui Ma
DOI: 10.1186/S13068-018-1150-8
关键词: Corn stover 、 Biochemistry 、 Hemicellulose 、 Saturated mutagenesis 、 Xylobiose 、 Thermostability 、 Lignocellulosic biomass 、 Xylanase 、 Chemistry 、 Biorefinery
摘要: … crystal structures of XYL10C-ΔN and its complex with xylobiose, Glu175 located on loop 3 was found to be specific to GH10 xylanases … of another two GH10 xylanases, XylE and XynE2, …
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Zbyszek Otwinowski, Wladek Minor, Processing of X-ray diffraction data collected in oscillation mode Methods in Enzymology. ,vol. 276, pp. 307- 326 ,(1997) , 10.1016/S0076-6879(97)76066-X
Veeresh Juturu, Jin Chuan Wu, Insight into microbial hemicellulases other than xylanases: a review. Journal of Chemical Technology & Biotechnology. ,vol. 88, pp. 353- 363 ,(2013) , 10.1002/JCTB.3969
Klaus Hantke, Volkmar Braun, Covalent binding of lipid to protein. Diglyceride and amide-linked fatty acid at the N-terminal end of the murein-lipoprotein of the Escherichia coli outer membrane. FEBS Journal. ,vol. 34, pp. 284- 296 ,(1973) , 10.1111/J.1432-1033.1973.TB02757.X
Shabir Najmudin, Benedita A Pinheiro, José AM Prates, Harry J Gilbert, Maria J Romão, Carlos MGA Fontes, None, Putting an N-Terminal End to the Clostridium Thermocellum Xylanase Xyn10B Story: Crystal Structure of the Cbm22-1-Gh10 Modules Complexed with Xylohexaose. Journal of Structural Biology. ,vol. 172, pp. 353- 362 ,(2010) , 10.1016/J.JSB.2010.07.009
Junqing Wang, Zhongbiao Tan, Minchen Wu, Jianfang Li, Jing Wu, Improving the thermostability of a mesophilic family 10 xylanase, AuXyn10A, from Aspergillus usamii by in silico design. Journal of Industrial Microbiology & Biotechnology. ,vol. 41, pp. 1217- 1225 ,(2014) , 10.1007/S10295-014-1463-Y
Kristin Rudolph, Peter Bauer, Benedikt Schmid, Frieder Mueller-Uri, Wolfgang Kreis, Truncation of N-terminal regions of Digitalis lanata progesterone 5β-reductase alters catalytic efficiency and substrate preference. Biochimie. ,vol. 101, pp. 31- 38 ,(2014) , 10.1016/J.BIOCHI.2013.12.010
Michael Widmann, Robert Radloff, Jürgen Pleiss, The Thiamine diphosphate dependent Enzyme Engineering Database: A tool for the systematic analysis of sequence and structure relations BMC Biochemistry. ,vol. 11, pp. 9- 9 ,(2010) , 10.1186/1471-2091-11-9
Letian Song, Claire Dumon, Béatrice Siguier, Isabelle André, Elena Eneyskaya, Anna Kulminskaya, Sophie Bozonnet, Michael Joseph O’Donohue, Impact of an N-terminal extension on the stability and activity of the GH11 xylanase from Thermobacillus xylanilyticus. Journal of Biotechnology. ,vol. 174, pp. 64- 72 ,(2014) , 10.1016/J.JBIOTEC.2014.01.004
Rajeev Kumar, Charles E. Wyman, Effect of xylanase supplementation of cellulase on digestion of corn stover solids prepared by leading pretreatment technologies. Bioresource Technology. ,vol. 100, pp. 4203- 4213 ,(2009) , 10.1016/J.BIORTECH.2008.11.057
Huiying Luo, Jiang Li, Jun Yang, Hui Wang, Yuhui Yang, Huoqing Huang, Pengjun Shi, Tiezheng Yuan, Yunliu Fan, Bin Yao, A thermophilic and acid stable family-10 xylanase from the acidophilic fungus Bispora sp. MEY-1 Extremophiles. ,vol. 13, pp. 849- 857 ,(2009) , 10.1007/S00792-009-0272-0