HIV-1 Recruits UPF1 but Excludes UPF2 to Promote Nucleocytoplasmic Export of the Genomic RNA.

摘要: Unspliced, genomic HIV-1 RNA (vRNA) is a component of several ribonucleoprotein complexes (RNP) during the viral replication cycle. In earlier work, we demonstrated that host upframeshift protein 1 (UPF1), key factor in nonsense-mediated mRNA decay (NMD), colocalized and associated to structural Gag egress. this demonstrate new function for UPF1 regulation vRNA nuclear export. OPEN ACCESS Biomolecules 2015, 5 2809 We establish nucleocytoplasmic shuttling required exists two essential RNPs late phase replication: first, export RNP contains Rev, CRM1, DDX3 nucleoporin p62, second, which excludes these markers but cytoplasm. Interestingly, observed both UPF2 long isoform UPF3a, UPF3aL, not shorter isoforms UPF3aS UPF3b, are excluded from UPF1-Rev-CRM1-DDX3 complex as they negative regulators silico protein-protein docking analyses suggest Rev binds region overlaps binding site, thus explaining exclusion regulatory by necessary trafficking. This work uncovers novel unique circuit involving UPF proteins ultimately regulate