Aldosterone-induced and GTP-stimulated methylation of a 90-kDa polypeptide in the apical membrane of A6 epithelia
作者： S Sariban-Sohraby , R S Fisher , M Abramow
DOI: 10.1016/S0021-9258(19)74356-7
关键词: Membrane protein 、 Biochemistry 、 Apical membrane 、 GTP' 、 Biology 、 Guanosine 、 Molecular biology 、 Aldosterone 、 Methylation 、 Membrane 、 Cell membrane
摘要: Aldosterone treatment of A6 cultured renal epithelial cells methylates the apical membrane, and we examined aldosterone-induced carboxymethylation membrane these to determine targeted polypeptides. Methionine-deprived were incubated with aldosterone [3H]methionine. Homogenates membranes solubilized analyzed by SDS-polyacrylamide gel electrophoresis. Label incorporation in a 90-kDa polypeptide was more intense (4-fold) after compared control. For vitro methylation, isolated, S-adenosyl-L-[methyl-3H]methionine, for 3H-methyl uptake. low control but markedly stimulated preparations from aldosterone-treated cells. Guanosine 5'-O-(3-thiotriphosphate) increased methylation 5-fold aldosterone, where already stimulated, little change observed. We conclude that induces an polypeptide, possibly subunit Na+ channel, GTP-dependent manner, this may be one final steps cascade reactions leading natriferic action hormone.
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sci-hub.st 参考文章(39)
S. Sariban-Sohraby, M. Abramow, R. S. Fisher, Single-channel behavior of a purified epithelial Na+ channel subunit that binds amiloride. American Journal of Physiology-cell Physiology. ,vol. 263, ,(1992) , 10.1152/AJPCELL.1992.263.5.C1111
D J Benos, G Saccomani, S Sariban-Sohraby, The epithelial sodium channel. Subunit number and location of the amiloride binding site. Journal of Biological Chemistry. ,vol. 262, pp. 10613- 10618 ,(1987) , 10.1016/S0021-9258(18)61007-5
Eric Lingueglia, Nicolas Voilley, Rainer Waldmann, Michel Lazdunski, Pascal Barbry, Expression cloning of an epithelial amiloride-sensitive Na+ channel: A new channel type with homologies to Caenorhabditis elegans degenerins FEBS Letters. ,vol. 318, pp. 95- 99 ,(1993) , 10.1016/0014-5793(93)81336-X
P S Backlund, R R Aksamit, Guanine nucleotide-dependent carboxyl methylation of mammalian membrane proteins. Journal of Biological Chemistry. ,vol. 263, pp. 15864- 15867 ,(1988) , 10.1016/S0021-9258(18)37528-8
T R Kleyman, E J Cragoe, J P Kraehenbuhl, The cellular pool of Na+ channels in the amphibian cell line A6 is not altered by mineralocorticoids. Analysis using a new photoactive amiloride analog in combination with anti-amiloride antibodies. Journal of Biological Chemistry. ,vol. 264, pp. 11995- 12000 ,(1989) , 10.1016/S0021-9258(18)80165-X
D.A. Ausiello, J.L. Stow, H.F. Cantiello, J.B. de Almeida, D.J. Benos, Purified epithelial Na+ channel complex contains the pertussis toxin-sensitive G alpha i-3 protein Journal of Biological Chemistry. ,vol. 267, pp. 4759- 4765 ,(1992) , 10.1016/S0021-9258(18)42897-9
T R Kleyman, D Landry, A Karlin, E Cragoe, C Ashbaugh, T Yulo, Q Al-Awqati, Photoaffinity labeling of the epithelial sodium channel. Journal of Biological Chemistry. ,vol. 261, pp. 2839- 2843 ,(1986) , 10.1016/S0021-9258(17)35863-5
F. Eckstein, D. Cassel, H. Levkovitz, M. Lowe, Z. Selinger, Guanosine 5'-O-(2-thiodiphosphate). An inhibitor of adenylate cyclase stimulation by guanine nucleotides and fluoride ions. Journal of Biological Chemistry. ,vol. 254, pp. 9829- 9834 ,(1979) , 10.1016/S0021-9258(19)83591-3
T R Kleyman, J P Kraehenbuhl, S A Ernst, Characterization and cellular localization of the epithelial Na+ channel. Studies using an anti-Na+ channel antibody raised by an antiidiotypic route. Journal of Biological Chemistry. ,vol. 266, pp. 3907- 3915 ,(1991) , 10.1016/S0021-9258(19)67880-4
S Sariban-Sohraby, E J Sorscher, B M Brenner, D J Benos, Phosphorylation of a single subunit of the epithelial Na+ channel protein following vasopressin treatment of A6 cells. Journal of Biological Chemistry. ,vol. 263, pp. 13875- 13879 ,(1988) , 10.1016/S0021-9258(18)68325-5