Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases.

摘要: The separation of ionized bases by reversed-phase liquid chromatography with alkyl silica columns often leads to severely tailed bands that are highly detrimental. Band shape and its dependence on sample mass notably different when mobile-phase pH is changed, this behavior has not been previously explained. Ionized silanols present in the stationary phase have credited a role determining peak shape. In study, separations two polymer were compared those obtained phases. Because absent from columns, comparison enabled us assess phases offer an explanation why band changes size for both silica-based