EXPLOITATION OF GENE(S) INVOLVED IN 2,4- DIACETYLPHLOROGLUCINOL BIOSYNTHESIS TO CONFER A NEW BIOCONTROL CAPABILITY TO A PSEUDOMONAS STRAIN

摘要: Tn5 mutagenesis and complementation analysis were used to clone a 6-kb genomic fragment required for biosynthesis of 2,4-diacetylphloroglucinol (Phl) from fluorescent Pseudomonas sp. strain F113. A recombinant plasmid, pCU203, containing this region partially complemented Phl production-negative mutant (F113G22) derived When sugar beet seeds sown into an unsterilized soil, in which was subject damping-off by Pythium ultimum, the emergence inoculated with F113 significantly greater than that F113G22. Transfer pCU203 eight other strains conferred ability synthesize only one these strains, M114. Strain M114(pCU203) showed enhanced antagonism towards P. ultimum vitro increased same soil compared induced parent