UGA can be decoded as tryptophan at low efficiency in Bacillus subtilis.

作者： P S Lovett , N P Ambulos , W Mulbry , N Noguchi , E J Rogers

DOI: 10.1128/JB.173.5.1810-1812.1991

关键词: Tryptophan 、 Genetic code 、 Biology 、 Bacillus subtilis 、 Bacillaceae 、 Mutagenesis (molecular biology technique) 、 Chloramphenicol Resistance 、 Nonsense mutation 、 Gene 、 Genetics

摘要: Replacement of cat-86 codon 7 or 144 with the UGA permitted gene to confer chloramphenicol resistance in wild-type Bacillus subtilis. UAA replacements same codons resulted a chloramphenicol-sensitive phenotype B. subtilis and chloramphenicol-resistant suppressor-positive strains. N-terminal sequencing showed that at was decoded as tryptophan cells, an efficiency about 6%. Images