Transport of [14C]hypoxanthine by sheep choroid plexus epithelium as a monolayer in primary culture: Na+-dependent and Na+-independent uptake by the apical membrane and rapid intracellular metabolic conversion to nucleotides.

摘要: Hypoxanthine is the main product of purine metabolic degradation and previous studies have revealed that it present in sheep CSF plasma micromolar concentrations. The aim this study was to elucidate transport molecule across choroid plexus epithelium (CPE) as a monolayer primary culture, explore mechanism uptake by apical side CPE investigate changes inside cell. estimated permeability for [14C]hypoxanthine, [14C]adenine [14C]guanine low comparable towards extracellular space markers. [14C]hypoxanthine two components: Na+-dependent Na+-independent, latter being partially mediated equilibrative nucleoside transporter 2. HPLC with simultaneous detection radioactivity majority metabolised into [14C]nucleotides [14C]inosine. remaining intact transported opposite, basolateral appeared lower chamber buffer together These findings indicate possible roles hypoxanthine from CP vivo: provide material nucleotide synthesis through salvage pathways CPE, well transfer excess blood.