Low vector competence in sylvatic mosquitoes limits Zika virus to initiate an enzootic cycle in South America

摘要: Zika virus (ZIKV) has spread in the Americas since 2015 and potential establishment of a sylvatic transmission cycle continent been hypothesized. We evaluated vector competence five Neotropical mosquito species to two ZIKV isolates. Distinct batches Haemagogus leucoceleanus, Sabethes albiprivus, identicus, Aedes terrens scapularis females were respectively orally challenged inoculated intrathoracically with ZIKV. Orally mosquitoes refractory or exhibited low infection rates. Viral dissemination was detected only Hg. leucocelaenus, but very Virus not saliva any ZIKV, regardless viral isolate incubation time. When injected, disseminated high rates Sa. identicus albpiprivus, these species; no Ae. scapularis. Together results suggest that genetically determined tissue barriers, especially midgut, play vital role inhibiting for tested species. Thus, an independent enzootic South America is unlikely.