Cellular deoxyribonucleotide triphosphate pools levels and DNA synthesis.

摘要: Abstract Growth curves were established for mouse 3T3 cells replicating on a plastic surface and L in suspension culture. At different stages of growth the analyzed deoxyribonucleoside triphosphate content exponential phase contained pools similar to those previously found embryo cells. When formed confluent layer, fraction synthesizing DNA was very small; such cultures dCTP, dGTP dTTP decreased while pool dATP remained approximately stationary. The decrease dCTP so marked that ratio between two orders magnitude. In exponentially culture unusually small, other types rapidly cell suspensions became dense but much less than observed with Also, changes levels limited compared cultures. Cellular four triphosphates also determined synchronized Chinese hamster obtained by mitotic selection or addition complete medium isoleucine-starved Using either technique synchrony high. Cells not involved synthesis significant triphosphates, expanded when proceeded through S cycle. all timepoints largest smallest amounting 1–5% size pool. closely correlated rate reached their highest values after synthesis.