Efficiency and fidelity of T3 DNA ligase in ligase-catalysed oligonucleotide polymerisations.

作者: Yi Lei , Joshua Washington , Ryan Hili

DOI: 10.1039/C8OB01958D

关键词: Computational biologyOligonucleotideFidelityDNA ligaseEnzymeContext (language use)DNA LigasesNucleic acidFunctional activityChemistry

摘要: … the efficiency of T3 DNA ligase and T4 DNA ligase for various … We find that T3 DNA ligase exhibits high efficiency and fidelity … The limitations and strengths of the two ligases are further …

参考文章(23)
Chun Guo, Christopher P. Watkins, Ryan Hili, Sequence-Defined Scaffolding of Peptides on Nucleic Acid Polymers. Journal of the American Chemical Society. ,vol. 137, pp. 11191- 11196 ,(2015) , 10.1021/JACS.5B07675
Stella Diafa, Marcel Hollenstein, Generation of Aptamers with an Expanded Chemical Repertoire. Molecules. ,vol. 20, pp. 16643- 16671 ,(2015) , 10.3390/MOLECULES200916643
Yi Lei, Dehui Kong, Ryan Hili, A High-Fidelity Codon Set for the T4 DNA Ligase-Catalyzed Polymerization of Modified Oligonucleotides ACS Combinatorial Science. ,vol. 17, pp. 716- 721 ,(2015) , 10.1021/ACSCOMBSCI.5B00119
Fabian Tolle, Gerhard M. Brändle, Daniel Matzner, Günter Mayer, A Versatile Approach Towards Nucleobase‐Modified Aptamers Angewandte Chemie. ,vol. 54, pp. 10971- 10974 ,(2015) , 10.1002/ANIE.201503652
Debra L. Robertson, Gerald F. Joyce, Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA Nature. ,vol. 344, pp. 467- 468 ,(1990) , 10.1038/344467A0
John A. Latham, Richard Johnson, John J. Toole, The application of a modified nucleotide in aptamer selection: novel thrombin aptamers containing 5-(1-pentynyl)-2'-deoxyuridine. Nucleic Acids Research. ,vol. 22, pp. 2817- 2822 ,(1994) , 10.1093/NAR/22.14.2817
John C Rohloff, Amy D Gelinas, Thale C Jarvis, Urs A Ochsner, Daniel J Schneider, Larry Gold, Nebojsa Janjic, Nucleic Acid Ligands With Protein-like Side Chains: Modified Aptamers and Their Use as Diagnostic and Therapeutic Agents. Molecular therapy. Nucleic acids. ,vol. 3, ,(2014) , 10.1038/MTNA.2014.49
Liang Cai, Changyun Hu, Shuiyuan Shen, Weirong Wang, Weida Huang, Characterization of Bacteriophage T3 DNA Ligase Journal of Biochemistry. ,vol. 135, pp. 397- 403 ,(2004) , 10.1093/JB/MVH047
Andrew D. Ellington, Jack W. Szostak, In vitro selection of RNA molecules that bind specific ligands. Nature. ,vol. 346, pp. 818- 822 ,(1990) , 10.1038/346818A0