Fate in soil of a recombinant plasmid carrying aDrosophila gene
作者： Monica A. Devanas , Guenther Stotzky
DOI: 10.1007/BF01568653
关键词: Biology 、 Hybridization probe 、 DNA 、 PBR322 、 Escherichia coli 、 Microbiology 、 Molecular biology 、 Complementary DNA 、 Plasmid 、 Recombinant DNA 、 Heterologous
摘要: A recombinant plasmid (C357; 3.5 Mdal) containing heterologous DNA (pBR322 [2.6 Mdal] with cDNA for an egg yolk protein fromDrosophila grimshawi) inEscherichia coli strain HB101 survived in and was recovered on selective media from sterile nonsterile soil during 27 days at frequencies similar to those of theE. coli(pBR322) system. In saline, the numbers all cells decreased 34 days, but plasmidless host declined less. There no loss either or as determined by colony hybridization a32P-labeled probe cDNA, HB101(C357) appeared be less able than HB101(pBR322) cope conditions starvation. These results suggested that nonessential eucaryotic inserted into has little effect survival saline bacterial maintenance vector.
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