Leishmanicidal activity of lipophilic extracts of some Hypericum species.

摘要: Abstract Leishmaniasis has emerged as the third most prevalent parasite-borne disease worldwide after malaria and filariasis, with about 350 million people at risk of infection. Antileishmanial drugs currently available have various limitations, mainly because parasite resistance side effects. The search new antileishmanial is ventured throughout world. Purpose purpose this study was to assess leishmanicidal activity lipophilic extracts eight Hypericum species against promastigote forms Leishmania (Leishmania) amazonensis. Material methods dried powered materials aerial parts H. andinum Gleason, brevistylum Choisy, caprifoliatum Cham. & Schltdl., carinatum Griseb., linoides A. St.-Hil., myrianthum polyanthemum Klotzsch ex Reichardt silenoides Juss. were extracted by static maceration n-hexane. Extracts evaporated dryness under reduced pressure stored −20 °C until biological evaluation HPLC analysis. metabolites investigated dimeric phloroglucinol derivatives, benzophenones benzopyrans. yields expressed mean three injections in mg compound per g extract (mg/g extract). effect on viability infective L. (L.) amazonensis determined using a hemocytometer. Amphotericin B used standard drug. 50% inhibitory concentration (IC50) values for each linear regression cytotoxic effects assessed peritoneal macrophages BALB/c mice MTT assay. that causes macrophage cytotoxicity (CC50) selectivity index (SI) considering following equation: CC50 mammalian cells/IC50 Results We demonstrated carinatum, able kill parasites dose dependent manner. These presented low murine macrophages. At 48 h incubation significant 36.1 µg/ml. significantly lower than polyanthemum, extracts. showed only high concentrations (500 1000 µg/ml), while ineffective. Conclusion promising results demonstrate importance genus source compounds potentially useful treatment leishmaniasis.