Changes in the plasma proteome of Manduca sexta larvae in relation to the transcriptome variations after an immune challenge: evidence for high molecular weight immune complex formation

摘要: Manduca sextais a lepidopteran model widely used to study insect physiological processes, including innate immunity. In this study, we explored the proteomes of cell-free hemolymph from larvae injected with sterile buffer (C for control) or mixture bacteria (I induced). Of 654 proteins identified, 70 showed 1.67 >200-fold abundance increases after immune challenge; 51 decreased 0-60% control levels. While there was no strong parallel between plasma protein levels and their transcript in hemocytes fat body, mRNA level changes (i.e.I/C ratios normalized read numbers) tissues concurred spectral counts) correlation coefficients 0.44 0.57, respectively. Better correlations support that body contributes more significant portion involved various aspects Consistently, were better correlated immunity-related than unrelated ones. There is set whose apparent molecular masses differ considerably calculatedMr's, suggestive posttranslational modifications. addition, some lowMrproteins detected range 80 >300 kDa on reducing SDS-polyacrylamide gel, indicating existence highMrcovalent complexes. We identified 30 serine proteases homologs, 11 which are known members an extracellular signaling network. Along our quantitative transcriptome data, identification, inducibility, association provide leads toward focused exploration humoral immunity inM. sexta.