Comparação de Meios de Enriquecimento e de Plaqueamento Utilizados na Pesquisa de Salmonella em Carcaças de Frango e Fezes de Aves
作者： MS Nascimento , A Berchieri Jr , MD Barbosa , FT Zancan , WAF Almeida
DOI: 10.1590/S1516-635X2000000100012
关键词: Salmonella 、 XLD agar 、 Biology 、 Serotype 、 Food science 、 Agar 、 Broiler 、 Brilliant green 、 Feces 、 Microbiology 、 Bacteria
摘要: This study was undertaken to compare selenite-cystine-novobiocin (SCN) broth, tetrationate-novobiocin (TN) broth and Rappaport-Vassiliadisnovobiocin (RVN) as enrichment MacConkey (MC) agar, brilliant green (BG) Hektoen (HE) Salmonella-Shigella (SS) agar xylose Iysine desoxycholate (XLD) plating media for isolating Salmonella from broiler carcasses chicken feces. The were washed with 300mL of Buffer Peptone Water 0.1% (BPW). BPW solation incubated 6h at ambient temperature up 24 h/43oC. From this, 2 mL inoculate in 20 43oC/24h. plated on the five tested selective agars, also Up colonies each plate inoculated TSI LIA 37oC/24h. genus confirmed by slide agglutination test using poly serum anti-somatic antigens (O) anti-fiagellar (H) antigens. feces experimentally contaminated eight serotypos (Agona, Anatum, Enteritidis, Havana, Intantis, Owakam, Schwazengrundand Typhimurium). Each serotype times. serotypes added sample individually so that final concentration bacterium 1,2 x iO3 ufclg. Two grams broth. proceduring this point wes same adopted examination. To there no statistic difference (p>0, 001) among media. However use two (SCN TN) agars between XLD SS HE gave bost results. assay done showed TN much better than others and, case it can be either or VB results examination is not easy determine bacteriologic routine search suggest association eorichments may improve test.
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bvs-vet.org.br参考文章(40)
Patricia S Sherrod, Rene Miguel Amaguana, Wallace H Andrews, Geraldine A June, Thomas S Hammack, Relative effectiveness of selective plating agars for recovery of Salmonella species from selected high-moisture foods. Journal of AOAC International. ,vol. 78, pp. 679- 690 ,(1995) , 10.1093/JAOAC/78.3.679
M. Mrđen, M. Gagrčin, M. Galić, D. Ćirković, Z. Mašić, Salmonella isolation from the organs of slaughter animals and fish meal. Veterinarski Glasnik. ,vol. 44, pp. 281- 285 ,(1990)
Welton I. Taylor, Dorothy Schelhart, Isolation of Shigellae. VII. Comparison of Gram-Negative Broth with Rappaport's Enrichment Broth Applied and Environmental Microbiology. ,vol. 18, pp. 393- 395 ,(1969) , 10.1128/AM.18.3.393-395.1969
C. N. Huhtanen, J. Naghski, Effect of Type of Enrichment and Duration of Incubation on Salmonella Recovery from Meat-and-Bone Meal Applied Microbiology. ,vol. 23, pp. 578- 585 ,(1972) , 10.1128/AM.23.3.578-585.1972
Welton I. Taylor, Dorothy Schelhart, Isolation of Shigellae Applied Microbiology. ,vol. 21, pp. 32- 37 ,(1971) , 10.1128/AM.21.1.32-37.1971
Sylvia King, William I. Metzger, A New Plating Medium for the Isolation of Enteric Pathogens Applied Microbiology. ,vol. 16, pp. 577- 578 ,(1968) , 10.1128/AM.16.4.577-578.1968
Welton I. Taylor, John H. Silliker, Isolation of Salmonellae from Foods Samples Applied Microbiology. ,vol. 9, pp. 484- 486 ,(1961) , 10.1128/AM.9.6.484-486.1961
Helen M. Pollock, Bonnie J. Dahlgren, Clinical Evaluation of Enteric Media in the Primary Isolation of Salmonella and Shigella Applied Microbiology. ,vol. 27, pp. 197- 201 ,(1974) , 10.1128/AM.27.1.197-201.1974
H. Bourhy, M. le Menec, M. Guittet, G. Bennejean, H. Morvan, C. Lahellec, Comparison of different enrichment and isolation media for Salmonella gallinarum pullorum. Recueil de Médecine Vétérinaire. ,vol. 166, pp. 793- 797 ,(1990)
A. A. Hajna, S. R. Damon, New Enrichment and Plating Media for the Isolation of Salmonella and Shigella Organisms1 Applied Microbiology. ,vol. 4, pp. 341- 345 ,(1956) , 10.1128/AM.4.6.341-345.1956