Effects of calcium phosphate/chitosan composite on bone healing in rats: calcium phosphate induces osteon formation.

摘要: Vascularization of an artificial graft represents one the most significant challenges facing field bone tissue engineering. Over past decade, strategies to vascularize scaffolds have been intensively evaluated using osteoinductive calcium phosphate (CaP) biomaterials in animal models. In this work, we observed that CaP-based implanted into rat calvarial defects showed remarkably accelerated formation and mineralization new woven initial stages, at a rate ∼60 μm/day (0.8 mg/day), which was considerably higher than normal growth rates (several μm/day, 0.1 mg/day) implant-free controls same age. Surprisingly, also histological evidence primary osteon formation, indicated by blood vessels early-region fibrous tissue, encapsulated lamellar osteocyte structures. These were later fully replaced compact bone, indicating complete regeneration bone. Thus, CaP biomaterial used here is not only osteoinductive, but vasculogenic, it may contributed regeneration, despite absence osteons calvaria. Further investigation will involve how strategy can regulate vascularized cortical such as control degradation rate, use models long, dense bones, more closely approximate repair human