The 3D architecture of a bacterial swarm has implications for antibiotic tolerance
作者： Jonathan D. Partridge , Gil Ariel , Orly Schvartz , Rasika M. Harshey , Avraham Be’er
DOI: 10.1038/S41598-018-34192-2
关键词: Cell Mobility 、 Bacteria 、 Chemotaxis 、 Biological system 、 Boundary value problem 、 Boundary (topology) 、 Chemistry 、 Swarming motility 、 Swarm behaviour 、 Multidrug tolerance 、 Multidisciplinary
摘要: Swarming bacteria are an example of a complex, active biological system, where high cell density and super-diffusive mobility confer survival advantages to the group as whole. Previous studies on dynamics swarm have been limited easily observable regions at advancing edge cells restricted plane. In this study, using defocused epifluorescence video imaging, we tracked motion fluorescently labeled individuals within interior densely packed three-dimensional (3D) region swarm. Our analysis reveals novel 3D architecture, constrained by inter-particle interactions, sandwiched between two distinct boundary conditions. We find that secreted biosurfactants keep away from swarm-air upper boundary, added antibiotics lower swarm-surface lead their migration boundary. Formation antibiotic-avoidance zone is dependent functional chemotaxis signaling in absence which loses its tolerance antibiotics.
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