Sprouty3 and Sprouty4, Two Members of a Family Known to Inhibit FGF-Mediated Signaling, Exert Opposing Roles on Proliferation and Migration of Glioblastoma-Derived Cells.

摘要: Dysregulation of receptor tyrosine kinase-induced pathways is a critical step driving the oncogenic potential brain cancer. In this study, we investigated role two members Sprouty (Spry) family in cancer-derived cell lines. Using immunoblot analyses found essential differences pattern endogenous Spry3 and Spry4 expression. While expression was mitogen-dependent repressed number cells from higher malignant cancers, levels neither fluctuated response to serum withdrawal nor were glioblastoma (GBM)-derived accordance well-known inhibitory Spry proteins fibroblast growth factor (FGF)-mediated signaling, both able interfere with FGF-induced activation MAPK pathway although different extent. solely, exerts its as negative regulator activation. Ectopic inhibited proliferation migration GBM-originated cells, positioning it tumor suppressor contrast, elevated accelerated these lines, while repression using shRNA caused significant diminished velocity rate GBM-derived line. This argues for tumor-promoting function GBMs. Based on data conclude that fulfill if not opposing roles within cancerogenesis malignancies.