Strains, functions and dynamics in the expanded Human Microbiome Project

摘要: The characterization of baseline microbial and functional diversity in the human microbiome has enabled studies microbiome-related disease, diversity, biogeography, molecular function. National Institutes Health Human Microbiome Project provided one broadest such characterizations so far. Here we introduce a second wave data from study, comprising 1,631 new metagenomes (2,355 total) targeting diverse body sites with multiple time points 265 individuals. We applied updated profiling assembly methods to provide personalization. Strain identification revealed subspecies clades specific sites; it also quantified species phylogenetic under-represented isolate genomes. Body-wide classified pathways into universal, human-enriched, site-enriched subsets. Finally, temporal analysis decomposed variation rapidly variable, moderately stable This study furthers our knowledge enables an understanding personalized function dynamics.