Transforming growth factor-β1: Comparative immunohistochemical localization in human primary and metastatic prostate cancer

摘要: BACKGROUND We have shown previously that primary prostate cancer demonstrates significant extracellular accumulation of transforming growth factor-beta 1 (TGF-beta 1). To further investigate the potential role TGF-beta in progression, we evaluated an expanded series prostatic carcinomas and associated lymph node metastases. EXPERIMENTAL DESIGN Prostate tissue samples from 37 patients were examined. Three organ donors, all less than 30 years age, whose prostates included as normal controls. Eighteen had undergone radical prostatectomy pelvic dissection. Eleven without evidence metastasis, whereas seven found to within at least one their nodes. Sixteen transurethral resection for benign disease, yet resected specimen (15 stage T1b; was T1a). Twelve with T1b disease underwent dissection implantation radioactive gold seeds. All 12 node. specimens examined level expression localization by immunohistochemistry using Ab distinguish intracellular 1. RESULTS Normal hyperplasia demonstrated negative or weak staining By comparison, 29 34 cancers showed extensive pronounced epithelial cells 13 patients. There no difference pattern between However, metastases, only 15 strong compared 19 tumors metastatic disease. In addition, 2 metastases staining, but contained CONCLUSIONS These findings confirm our previous observation exhibits enhanced relative hyperplasia. study documented a significantly more metastasis metastasis. Moreover, although observed tumor is maintained lack site noted. This differential may be biologically important could conceivably reflect progression.