Ultra High-Resolution In vivo Computed Tomography Imaging of Mouse Cerebrovasculature Using a Long Circulating Blood Pool Contrast Agent.

摘要: Abnormalities in the cerebrovascular system play a central role many neurologic diseases. The on-going expansion of rodent models human diseases and need to use these understand disease progression treatment has amplified for reproducible non-invasive imaging methods high-resolution visualization complete cerebral vasculature. In this study, we present vivo (19 μm isotropic) computed tomography mouse brain This technique enabled 3D large networks, including Circle Willis. Blood vessels as small 40 μm were clearly delineated. ACTA2 mutations humans cause defects, abnormally straightened arteries moyamoya-like arteriopathy characterized by bilateral narrowing internal carotid artery stenosis arteries. studies performed model Acta2 demonstrated utility method studying vascular morphometric changes that are practically impossible identify using current histological methods. Specifically, width Willis, straightening arterial stenoses. We believe described here will contribute substantially study cerebrovasculature.