Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
作者： Sara Arana Peña , Nathalia Saraiva Rios , Carmen Mendez Sanchez , Yuliya Lokha , Luciana R.B. Gonçalves
DOI: 10.1016/J.ENZMICTEC.2020.109535
关键词: Glutaraldehyde 、 Chromatography 、 Candida antarctica 、 Agarose 、 Enzyme assay 、 Enzyme 、 Chemistry 、 Rhizomucor miehei 、 Substrate (chemistry) 、 Polyethylenimine
摘要: … The enzymes were initially immobilized via interfacial activation on octyl-agarose beads, treated with polyethylenimine (PEI) and a new enzyme layer was immobilized on the octyl-…
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