Techniques, Theory, and Biological Applications of Optically Detected Magnetic Resonance (ODMR)

摘要: The past dozen years or so have witnessed the application of a new spectroscopic method to investigation biological molecules. Optical detection triplet-state magnetic resonance (ODMR) represents in broadest sense combination optical measurement (phosphorescence, fluorescence, absorption) with electron spin (ESR) spectroscopy. In ODMR, microwave-induced transitions between sublevels lowest triplet state are detected by their effect on some property sample which is monitored simultaneously. Historically, field may be traced back early work atoms and ions gas phase Fermi Rasetti (1925), Breit Ellet Brossel Bitter (1952). first optically experiment solid was reported Geschwind et al. (1959) 2E excited Cr3+ ruby at 1.6 K. This has been reviewed Bernheim (1965). At about time Geschwind’s experiment, Hutchinson Mangum (1958; 1961) made ESR measurements photoexcited state—the phosphorescent (T 1) naphthalene substitutionally incorporated single crystal durene.