Biological characteristics of Clostridium perfringens type A enterotoxin.

摘要: An enterotoxin with the ability to induce fluid accumulation in rabbit ileal loops, erythema skin of guinea pigs, and lethality mice appears cell extracts (CE) culture filtrates (CF) sporulating cells some Clostridium perfringens type A strains. All activities CE CF were eluted simultaneously from a Sephadex G-200 column. Different elution patterns obtained for these present CF. Rabbit immune serum against active fractions eliminated three biological These not by any known antitoxins diagnostic C. types A, B, C, D, E. Immunodiffusion studies indicated precipitin line identity between NCTC 8798 other enterotoxin-positive strains but enterotoxin-negative Disc electrophoresis on 7.0% polyacrylamide gels revealed single area containing erythemal activity mouse lethality. acrylamide gels, crude fractionated enterotoxin, partially purified band same as activities. Immunoelectrophoresis also showed one which occurred only findings suggest that component is responsible attributed enterotoxin. Images