HUMAN PREGNANCY FOLLOWING CRYOPRESERVATION, THAWING AND TRANSFER OF AN EIGHT-CELL EMBRYO

作者: Alan Trounson , Linda Mohr

DOI: 10.1038/305707A0

关键词: AnatomyIn vitro fertilisationAndrologyEmbryo transferEmbryoEmbryo cryopreservationGestationPregnancyBiologyPregnancy rateCryopreservation

摘要: The widespread use of clomiphene citrate and exogenous gonadotrophins for in vitro fertilization (IVF) human frequently results the production multiple embryos. Replacement more than two embryos increases pregnancy rate but may result pregnancies with increased pre- post-natal abnormality. Preservation a limited time allows fewer to be replaced on several different occasions thus problems can minimized, effectiveness single IVF procedure embryo replacement adverse maternal conditions avoided. Preimplantation have been successfully cryopreserved many animal species. sensitivity cooling freezing varies between species stages development. We report here cryopreservation procedures that allow high survival four- eight-cell establishment following storage an 4 months liquid nitrogen. terminated at 24 weeks' gestation due development septic Streptomyces agalactiae chorion amnionitis after premature membrane rupture.

参考文章(13)
A. O. Trounson, B. F. Shea, G. W. Ollis, M. E. Jacobson, Frozen Storage and Transfer of Bovine Embryos Journal of Animal Science. ,vol. 47, pp. 677- 681 ,(1978) , 10.2527/JAS1978.473677X
V. H. T. James, Luciano Martini, Current topics in experimental endocrinology Academic Press. ,(1971)
John Leeton, Alan Trounson, David Jessup, Carl Wood, The technique for human embryo transfer Fertility and Sterility. ,vol. 38, pp. 156- 161 ,(1982) , 10.1016/S0015-0282(16)46451-4
A. O. Trounson, L. R. Mohr, C. Wood, J. F. Leeton, Effect of delayed insemination on in-vitro fertilization, culture and transfer of human embryos Reproduction. ,vol. 64, pp. 285- 294 ,(1982) , 10.1530/JRF.0.0640285
A. Trounson, J. Leeton, C Wood, J Webb, J Wood, Pregnancies in humans by fertilization in vitro and embryo transfer in the controlled ovulatory cycle. Science. ,vol. 212, pp. 681- 682 ,(1981) , 10.1126/SCIENCE.7221557
A Trounson, A Conti, Research in human in-vitro fertilisation and embryo transfer. BMJ. ,vol. 285, pp. 244- 248 ,(1982) , 10.1136/BMJ.285.6337.244
D. G. WHITTINGHAM, Survival of mouse embryos after freezing and thawing. Nature. ,vol. 233, pp. 125- 126 ,(1971) , 10.1038/233125A0
C. Wood, J. Leeton, J. Mc. Talbot, A. O. Trounson, TECHNIQUE FOR COLLECTING MATURE HUMAN OOCYTES FOR IN VITRO FERTILIZATION British Journal of Obstetrics and Gynaecology. ,vol. 88, pp. 756- 760 ,(1981) , 10.1111/J.1471-0528.1981.TB01279.X