Phenotypic consequences of purine nucleotide imbalance in Saccharomyces cerevisiae.

摘要: Coordinating homeostasis of multiple metabolites is a major task for living organisms, and complex interconversion pathways contribute to achieving the proper balance metabolites. AMP deaminase (AMPD) such an enzyme that allows IMP synthesis from AMP. In this article, we show that, under specific conditions, lack AMPD activity impairs growth. Under these found intracellular guanylic nucleotide pool was severely affected. vivo studies two homologs, Yjl070p Ybr284p, indicate proteins have no detectable AMP, adenosine, or adenine activity; overexpression YJL070c instead mimics loss function. Expression yeast transcriptome monitored in AMPD-deficient mutant strain overexpressing cells treated with immunosuppressive drug mycophenolic acid, three conditions lead severe depletion pool. These resulted up- downregulation transcripts, 244 which are common at least 71 all conditions. results, combined analysis, point threonine metabolism as exquisitely sensitive purine balance.