Upregulation of microRNA-218 reduces cardiac microvascular endothelial cells injury induced by coronary artery disease through the inhibition of HMGB1.

摘要: This study is performed to examine the impacts of microRNA-218 (miR-218) on cardiac microvascular endothelial cells (CMECs) injury induced by coronary artery disease (CAD). Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) was applied for detecting miR-218 expression in serum patients with CAD and healthy controls, correlation between clinical indexes such as creatine kinase, kinase-myocardial band, troponin I, Gensini score analyzed. CMECs were coincubated homocysteine 24 hr injury, transfected mimics or inhibitors. Besides, we used oxidized low density lipoprotein an inducer incubate hr, model established be mimics. RT-qPCR western blot analysis detect HMGB1 CMECs. A series experiments determine cell proliferation, apoptosis, migration, angiogenesis ability Vascular growth factor inflammatory contents measured. The obtained results suggested that peripheral blood descended substantially versus controls. Low found CAD-induced injury. Overexpressed promoted ability, alleviated may negatively regulate CAD. demonstrates upregulation reduces through inhibition HMGB1.