Metabolic profiling of Candida clinical isolates of different species and infection sources.

摘要: Candida species are the most common cause of opportunistic fungal infections. Rapid identification and novel approaches for characterization these fungi great interest to improve diagnosis knowledge about their pathogenic properties. This study aimed characterize clinical isolates spp. by proteomics (MALDI-TOF MS) metabolomics (1H-NMR), correlate metabolic profiles with species, source infection different virulence associated parameters. In particular, 49 strains from sources (blood, n = 15; vagina, n = 18; respiratory tract, n = 16), belonging mainly C. albicans complex (61%), glabrata (20%) parapsilosis (12%) were used. Several extracellular intracellular metabolites showed significantly concentrations among recovered infection, as well species. These related glycolysis or gluconeogenesis, tricarboxylic acid cycle, nucleic synthesis amino lipid metabolism. Moreover, we found specific fingerprints ability form biofilm, antifungal resistance (i.e. caspofungin fluconazole) production secreted aspartyl proteinase. conclusion, 1H-NMR-based can be useful deepen pathogenicity