The pathogenic potential of the combined action of chronic Opisthorchis felineus infection and repeated social defeat stress in C57BL/6 mice.

摘要: Abstract Parasitic food-borne diseases and chronic social stress are frequent attributes of day-to-day human life. Therefore, our aim was to model the combined action Opisthorchis felineus infection repeated defeat in C57BL/6 mice. Histological examination liver revealed inflammation sites, pronounced periductal fibrosis, cholangiofibrosis together with proliferation bile ducts hepatocyte dystrophy infected mice, especially stress-exposed ones. Simultaneously pathology, we detected significant structural changes cerebral cortex. Immunohistochemical analysis hippocampus indicated highest increase numerical density Iba 1-, IL-6-, iNOS-, Arg1-positive cells mice simultaneously subjected two adverse factors. The number GFAP-positive rose during stress, most strongly both stress. Real-time PCR showed that expression genes Aif1 Il6 differed among analysed brain regions (hippocampus, hypothalamus, frontal cortex) depended on factors applied. In addition, regions, there no consistent or decrease these parameters when treatments were combined: (i) hippocampus, upregulation change expression; (ii) levels not different from controls; (iii) cortex, did while increased. It can be concluded a combination long-lasting factors, O. worsens only hepatic but also state, as evidenced behaviorally by disturbances startle response