A Novel Action of Collapsin: Collapsin-1 Increases Antero- and Retrograde Axoplasmic Transport Independently of Growth Cone Collapse

摘要: Chick collapsin-1, a member of the by mechanism distinct from that causing growth semaphorin family, has been implicated in axonal cone collapse. Pertussis toxin (PTX) but not its B pathfinding as repulsive guidance cue. Collapsin-1 induces collapse via pathway which oligomer blocks collapsin-induced col- may include CRMP-62 and heterotrimeric G proteins. lapse. The holotoxin does affect collapsin-stimu- protein is related to UNC-33, nematode lated axoplasmic transport. Mastoparan myelin neuronal required for appropriately directed NI-35 induce PTX-sensitive extension. Mutations unc-33 neural lapse do stimulate microtubules, basic cytoskeletal elements axo- These results provide evidence collapsin plasmic Using computer-assisted video-en- unique property activate vesicular transport hanced differential interference contrast microscopy, systems. There are at least two pathways we now demonstrate collapsin-1 potently pro- through exerts actions motes doubles developing neurons. q 1997 John Wiley & Sons, Inc. J number antero- retrograde-transported organ- Neurobiol 33: 316-328, elles their velocity. decreases Keywords: collapsin; transport; nerve stationary organelles, suggesting cone; pertussis toxin; proteins fraction time during particle moving