Permeabilization of yeast cells: application to study on the regulation of AMP deaminase activity in situ.
作者： Keiko Murakami , Hiroshi Nagura , Masataka Yoshino
DOI: 10.1016/0003-2697(80)90479-0
关键词: In vitro 、 Enzyme 、 Yeast 、 AMP deaminase 、 Intracellular 、 Saccharomyces cerevisiae 、 Allosteric regulation 、 Biology 、 AMP deaminase activity 、 Biochemistry
摘要: Abstract A permeabilization method which allows the assay of several intracellular enzymes within boundaries yeast cell wall is described. Toluene treatment was found to make cells completely permeable exogenous substrates, and did not leak out treated cells. This also compared with techniques reported previously. Electron microscopic examination toluene-treated indicated that they were essentially intact. The kinetic properties AMP deaminase, examined in permeabilized cells, including allosteric regulation by polyamine Zn2+, suggest some differences protein interactions for deaminase situ vitro.
elsevier.com
sci-hub.se 参考文章(22)
R. L. Peterson, C. W. Radcliffe, N. R. Pace, Ribonucleic Acid Synthesis in Bacteria Treated with Toluene1 Journal of Bacteriology. ,vol. 107, pp. 585- 588 ,(1971) , 10.1128/JB.107.2.585-588.1971
Edvardas Kaminskas, Boris Magasanik, Sequential Synthesis of Histidine-degrading Enzymes in Bacillus subtilis Journal of Biological Chemistry. ,vol. 245, pp. 3549- 3555 ,(1970) , 10.1016/S0021-9258(18)62961-8
D.H. Levin, M.N. Thang, M. Grunberg-Manago, SYNTHESIS IN VIVO OF POLYNUCLEOTIDE PHOSPHORYLASE IN ESCHERICHIA COLI. I. EFFECT OF AMINO ACIDS ON POLYNUCLEOTIDE PHOSPHORYLASE ACTIVITY IN A CHLORAMPHENICOL-INHIBITED SYSTEM. Biochimica et Biophysica Acta. ,vol. 76, pp. 558- 571 ,(1963) , 10.1016/0926-6550(63)90082-3
M Swissa, H Weinhouse, M Benziman, Activities of citrate synthase and other enzymes of Acetobacter xylinum in situ and in vitro Biochemical Journal. ,vol. 153, pp. 499- 501 ,(1976) , 10.1042/BJ1530499
Keiko MURAKAMI, AMP Deaminase from Baker′s Yeast--Kinetic and Molecular Properties Journal of Biochemistry. ,vol. 86, pp. 1331- 1336 ,(1979) , 10.1093/OXFORDJOURNALS.JBCHEM.A132649
R. E. Alper, J. L. Dainko, F. Schlenk, Properties of Yeast Cell Ghosts Obtained by Ribonuclease Action Journal of Bacteriology. ,vol. 93, pp. 759- 765 ,(1967) , 10.1128/JB.93.2.759-765.1967
Ramon Serrano, Juana M. Gancedo, Carlos Gancedo, Assay of yeast enzymes in situ. A potential tool in regulation studies. FEBS Journal. ,vol. 34, pp. 479- 482 ,(1973) , 10.1111/J.1432-1033.1973.TB02783.X
Murray P. Deutscher, Preparation of Cells Permeable to Macromolecules by Treatment with Toluene: Studies of Transfer Ribonucleic Acid Nucleotidyltransferase Journal of Bacteriology. ,vol. 118, pp. 633- 639 ,(1974) , 10.1128/JB.118.2.633-639.1974
Masataka Yoshino, Keiko Murakami, Keizo Tusushima, Inhibition of AMP deaminase by zinc ions. Biochemical Pharmacology. ,vol. 27, pp. 2651- 2652 ,(1978) , 10.1016/0006-2952(78)90343-X
Richard E. Reeves, Alberto Sols, Regulation of Escherichia coli phosphofructokinase in situ Biochemical and Biophysical Research Communications. ,vol. 50, pp. 459- 466 ,(1973) , 10.1016/0006-291X(73)90862-0