The glutamate receptor 2 subunit controls post-synaptic density complexity and spine shape in the dentate gyrus.

摘要: In adult brain the majority of AMPA glutamate receptor (GluR) subunits contain GluR2. knock-out (KO) mice absence GluR2 results in consequences for synaptic plasticity including cognitive impairments. Here morphology dendritic spines and their contacts was analysed via three-dimensional reconstruction serial electron micrographs from dentate gyrus (DG) wild type (WT) KO mice. Pre-embedding immunocytochemical staining used to examine distribution subcellular localization GluR1 N-methyl-D-aspartate NR1 subunits. There were no significant changes synapse density DG compared with WT However, there a decrease percentage synapses on mushroom but an increase thin spines. also large proportion complex perforated/segmented post-synaptic densities (PSDs) (25 vs. 78% WT) macular PSDs (75 22%). These data coupled decreases volume surface area PSDs. both mice, receptors present dendrites reduction labelling spine membranes cytoplasm small immunolabelling Our demonstrate that has effect cytoarchitecture expression receptors.