Dielectric spectroscopy in a micromachined flow cytometer: theoretical and practical considerations

摘要: We propose a model to determine the influence of different cell properties, such as size, membrane capacitance and cytoplasm conductivity, on impedance spectrum measured in microfabricated cytometer. A dielectric sphere equivalent complex permittivity is used simplified describe biological cell. The measurement takes place between pair facing microelectrodes microchannel filled with saline solution. incorporates various parameters, size position channel. 3D finite element evaluate magnitude electric field channel resultant changes charge densities at electrode boundaries flows past. density integrated surface displacement current for computed frequency range. complete combines model, electrode–electrolyte interface stray impedance, which are from real device. modeled spectra parameters discussed strategy discrimination system proposed. finally discuss amount noise fluctuations sensor.