Density Gradient Centrifugation for the Separation of Sporulating Forms of Bacteria

摘要: Urografin has been shown to be suitable as a supporting medium for isopycnic density centrifugation of Bacillus megaterium spores. The complete separation vegetative cells and spores can achieved through the use such gradients. Vegetative were found have an average 1.135 g per cm3, while spore was 1.305 cm3. Under normal sporulating conditions formed consisted heterogeneous population with wide spectrum densities. These differences could correlated properties resistance thermal death ability heat-activated. Spores in absence Ca++ are less dense than those obtained under usual conditions. pattern cell distribution on gradient serve gauge efficiency sporulation degree synchrony culture.