THE INHIBITION OF RIBONUCLEIC ACID SYNTHESIS IN MAMMALIAN CELLS BY ACTINOMYCIN D.
作者： Richard M. Franklin
DOI: 10.1016/0926-6550(63)90379-7
关键词: Protoplasm 、 DNA 、 Biochemistry 、 Molecular biology 、 Nucleoprotein 、 Tritium 、 Metabolism 、 Phosphatase 、 Biology 、 Ribosomal RNA 、 RNA
摘要: Abstract Actinomycin D, a specific inhibitor of DNA-dependent RNA synthesis, will inhibit up to 99% all synthesis in mammalian cells. This inhibition has been studied detail. After exposure low doses actinomycin, ribosomal (28 and 18-S components) was completely inhibited soluble (4-S component) partially inhibited. With increasing concentrations incorporation label into 4-S more more. It not possible, however, suppress this completely. residual due the -CpCpA terminal bases souluble RNA. possible account for presence actinomycin fashion. Thus complete species by is demonstrated, proving that normal cells directed DNA templates.
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