Molecular studies of the Oka varicella vaccine

摘要: Varicella zoster virus (VZV) is one of eight members the Herpesviridae family for which humans are primary host; it causes two distinct diseases, varicella (chickenpox) and (shingles). results from infection, during establishes latency in sensory neurons, a characteristic all Alphaherpesvirinae subfamily. Zoster caused by reactivation latent virus, typically occurs when cellular immunity impaired. VZV first human herpesvirus vaccine has been licensed. The preparation, v-Oka, live-attenuated stock produced classic method tissue culture passage animal cell lines. Over 90 million doses have administered countries worldwide, including USA, where morbidity mortality declined dramatically. last decade, several laboratories committed to investigating mechanism Oka attenuated. Mutations accumulated across genome attenuation process; however, studies contribution these changes hampered lack suitable model disease heterogeneity that exists among viral population within preparation. Notwithstanding, wealth data generated using various laboratory methodologies. Studies xenografts implanted severe combined immunodeficiency-hu mice, enabled analyses replication dynamics dorsal root ganglia, T lymphocytes skin. In vitro assays used investigate effect mutations on gene expression sequence analysis rash viruses permitted investigations into spread host. We present here review what learned thus far about molecular phenotypic characteristics vaccine.