The development and evaluation of a nested PCR assay for detection of Neospora caninum and Hammondia heydorni in feral mouse tissues.

摘要: The development of a novel nested polymerase chain reaction is described and used for detecting the presence Neospora caninum Hammondia heydorni DNA in extracted from feral rodent tissues. A unique strategy was design an assay that could be adapted more than one member Toxoplasmatinae simultaneously with minimal number additional steps. level sensitivity this comparable to real time-PCR other PCR assays. Twenty-eight 104 mice tested positive N. at least tissue (the brain, heart or liver) studied. In study, eight instances are reported where brain negative while positive. This suggests prior studies, which screened only describe prevalence levels under-represented. None 54 mouse brains H. DNA. rarely infected by although hypothesis needs explored further. Data obtained current study suggest common parasite rodents may important epidemiology disease.