Rigid cyanine dye nucleic acid labels.

作者: Adrian Fegan , Pravin S. Shirude , Shankar Balasubramanian

DOI: 10.1039/B801629A

关键词: CarbocyaninesDNAChemistryPhotochemistryFörster resonance energy transferNucleic acidLinkerFluorescenceMoleculeCyanineCombinatorial chemistry

摘要: Cyanine dyes attached to DNA via a rigid linker show useful fluorescence and FRET properties without altering the stability of duplex DNA.

参考文章(14)
Lynda J Brown, Jonathan P May, Tom Brown, Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides Tetrahedron Letters. ,vol. 42, pp. 2587- 2591 ,(2001) , 10.1016/S0040-4039(01)00224-6
Hong Yu, Jean Chao, David Patek, Ratan Mujumdar, Swati Mujumdar, Alan S. Waggoner, Cyanine dye dUTP analogs for enzymatic labeling of DNA probes Nucleic Acids Research. ,vol. 22, pp. 3226- 3232 ,(1994) , 10.1093/NAR/22.15.3226
Stefan Jäger, Goran Rasched, Hagit Kornreich-Leshem, Marianne Engeser, Oliver Thum, Michael Famulok, A Versatile Toolbox for Variable DNA Functionalization at High Density Journal of the American Chemical Society. ,vol. 127, pp. 15071- 15082 ,(2005) , 10.1021/JA051725B
Janez Barbaric, Hans-Achim Wagenknecht, DNA as a supramolecular scaffold for the helical arrangement of a stack of 1-ethynylpyrene chromophores Organic and Biomolecular Chemistry. ,vol. 4, pp. 2088- 2090 ,(2006) , 10.1039/B605251G
Guan-Sheng Jiao, Taeg Gyum Kim, Michael R. Topp, Kevin Burgess, A blue-to-red energy-transfer thymidine analogue that functions in DNA. Organic Letters. ,vol. 6, pp. 1701- 1704 ,(2004) , 10.1021/OL049684W
Dennis J. Hurley, Yitzhak Tor, Ru(II) and Os(II) nucleosides and oligonucleotides: Synthesis and properties Journal of the American Chemical Society. ,vol. 124, pp. 3749- 3762 ,(2002) , 10.1021/JA0123103
Shoeb I. Khan, Mark W. Grinstaff, Palladium(0)-Catalyzed Modification of Oligonucleotides during Automated Solid-Phase Synthesis Journal of the American Chemical Society. ,vol. 121, pp. 4704- 4705 ,(1999) , 10.1021/JA9836794
Stephen J. Mason, Jen L. Hake, James Nairne, W. Jon Cummins, Shankar Balasubramanian, Solid-Phase Methods for the Synthesis of Cyanine Dyes Journal of Organic Chemistry. ,vol. 70, pp. 2939- 2949 ,(2005) , 10.1021/JO0479415
David G. Norman, Richard J. Grainger, Dusan Uhrín, David M. J. Lilley, Location of cyanine-3 on double-stranded DNA: importance for fluorescence resonance energy transfer studies. Biochemistry. ,vol. 39, pp. 6317- 6324 ,(2000) , 10.1021/BI992944A
Duncan Graham, John A. Parkinson, Tom Brown, DNA duplexes stabilized by modified monomer residues: synthesis and stability Journal of The Chemical Society-perkin Transactions 1. ,vol. 1998, pp. 1131- 1138 ,(1998) , 10.1039/A707031D